Submit MIxS Metadata

The INSDC databases

The International Nucleotide Sequence Database Collaboration (INSDC; NCBI/GenBank, EBI-ENA, and the DNA Databank of Japan), partners have recognised the MIxS, have reserved an official keyword for compliant INSDC sequence records in the form of “GSC:MIxS;{specific_checklist_name}”.

NCBI offers customizable templates to download for all MIxS checklists and the environmental packages in BioSample submissions. The BioSample concept fits very well with MIxS metadata, as our focus is also on the sample.

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ENA also offers customizable MIxS templates for downloads in their login-based submission system. “Submitting Environmental Sequences” and “MIxS” pages detail the submission process further.


The GOLD Database

The Genomes Online Database (GOLD) displays a wide range of metadata for complete and ongoing genome and metagenome projects. It now also accepts submission of new entries and metadata.


MG-RAST has implemented the use of MIxS by using simple spreadsheets to capture metadata, with a minimal number of required fields (in red in the spreadsheets) and a number of optional fields. The spreadsheet is separated into multiple tabs representing the different metadata categories. A more detailed explanation can be found in the MG-RAST blog.